Search results for "Yeast cells"
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An Off-On-Off Fluorescent Sensor for pH Windows Based on the 13aneN4-Zn 2+ System
2016
International audience; The new ligand L was prepared and features a 13-membered tetraaza macrocyclic ring with a 1,8-naphthalimide fluorophore appended to a C atom of its backbone. The protonation constants of L as well as its complexation constants with Zn2+ ions were determined in 1:1 water/methanol solutions by potentiometric titrations. Fluorimetric pH titrations were performed with L alone and L in the presence of Zn2+ ions (1:1), and the species distributions (%) versus pH were compared. A window-shaped fluorescence trend was observed with pH for the L/Zn2+ system, which behaves as an off-on-off pH sensor. The on window is centred in the 6.5-7.5 pH range, in correspondence with the f…
Can pressure-induced cell inactivation be related to cell volume compression? A case study for Saccharomyces cerevisiae
2013
In this paper, emphasis has been put on the relationship between volume compression and cell inactivation for the Saccharomyces cerevisiae strain CBS 1171 submitted to high hydrostatic pressure treatments. The influence of cell dehydration on pressure inactivation was first investigated. Inactivation was found to be strongly limited, or even completely prevented for cells with a water content of 60% w/w or below. Moreover, the volume compression undergone by a single yeast cell was assessed as a function of pressure and hydration conditions using a high-pressure setup for pressure-volume-temperature measurements. Direct measurements of volume compression were performed on cell pellets after…